@Article{Wright_Georg-Workf_for_Compu-2018, author = {Wright, Patrick R. and Georg, Jens}, title = {Workflow for a {Computational} {Analysis} of an {sRNA} {Candidate} in {Bacteria}}, journal = {Methods Mol Biol}, year = {2018}, volume = {1737}, number = {}, pages = {3-30}, user = {mmann}, pmid = {29484584}, doi = {10.1007/978-1-4939-7634-8_1}, issn = {1940-6029}, issn = {1064-3745}, abstract = {Computational methods can often facilitate the functional characterization of individual sRNAs and furthermore allow high-throughput analysis on large numbers of sRNA candidates. This chapter outlines a potential workflow for computational sRNA analyses and describes in detail methods for homolog detection, target prediction, and functional characterization based on enrichment analysis. The cyanobacterial sRNA IsaR1 is used as a specific example. All methods are available as webservers and easily accessible for nonexpert users.} }