@Article{Wecker_Hoffmeier_Plotner-Micro_Profi_Aqueo-2016,
  author =	 {Wecker, Thomas and Hoffmeier, Klaus and Plotner, Anne and 
                  Gruning, Bjorn Andreas and Horres, Ralf and Backofen, Rolf 
                  and Reinhard, Thomas and Schlunck, Gunther},
  title =	 {{MicroRNA} {Profiling} in {Aqueous} {Humor} of {Individual} 
                  {Human} {Eyes} by {Next}-{Generation} {Sequencing}},
  journal =	 {Invest Ophthalmol Vis Sci},
  year =	 {2016},
  volume =	 {57},
  number =	 {4},
  pages =	 {1706-13},
  user =	 {backofen},
  pmid =	 {27064390},
  doi = 	 {10.1167/iovs.15-17828},
  issn = 	 {0146-0404},
  issn = 	 {1552-5783},
  abstract =	 {PURPOSE: Extracellular microRNAs (miRNAs) in aqueous humor 
                  were suggested to have a role in transcellular signaling and 
                  may serve as disease biomarkers. The authors adopted 
                  next-generation sequencing (NGS) techniques to further 
                  characterize the miRNA profile in single samples of 60 to 80 
                  muL human aqueous humor. METHODS: Samples were obtained at 
                  the outset of cataract surgery in nine independent, 
                  otherwise healthy eyes. Four samples were used to extract 
                  RNA and generate sequencing libraries, followed by an 
                  adapter-driven amplification step, electrophoretic size 
                  selection, sequencing, and data analysis. Five samples were 
                  used for quantitative PCR (qPCR) validation of NGS results. 
                  Published NGS data on circulating miRNAs in blood were 
                  analyzed in comparison. RESULTS: One hundred fifty-eight 
                  miRNAs were consistently detected by NGS in all four 
                  samples; an additional 59 miRNAs were present in at least 
                  three samples. The aqueous humor miRNA profile shows some 
                  overlap with published NGS-derived inventories of 
                  circulating miRNAs in blood plasma with high prevalence of 
                  human miR-451a, -21, and -16. In contrast to blood, miR-184, 
                  -4448, -30a, -29a, -29c, -19a, -30d, -205, -24, -22, and 
                  -3074 were detected among the 20 most prevalent miRNAs in 
                  aqueous humor. Relative expression patterns of miR-451a, 
                  -202, and -144 suggested by NGS were confirmed by qPCR. 
                  CONCLUSIONS: Our data illustrate the feasibility of miRNA 
                  analysis by NGS in small individual aqueous humor samples. 
                  Intraocular cells as well as blood plasma contribute to the 
                  extracellular aqueous humor miRNome. The data suggest 
                  possible roles of miRNA in intraocular cell adhesion and 
                  signaling by TGF-beta and Wnt, which are important in 
                  intraocular pressure regulation and glaucoma.}
}