@Article{Tripp_Martin_Orell-Plast_archa_box-2016,
  author =	 {Tripp, Vanessa and Martin, Roman and Orell, Alvaro and 
                  Alkhnbashi, Omer S. and Backofen, Rolf and Randau, Lennart},
  title =	 {Plasticity of archaeal {C}/{D} box {sRNA} biogenesis},
  journal =	 {Mol Microbiol},
  year =	 {2016},
  volume =	 {103},
  number =	 {1},
  pages =	 {151-164},
  user =	 {alkhanbo},
  pmid =	 {27743417},
  doi = 	 {10.1111/mmi.13549},
  issn = 	 {0950-382X},
  issn = 	 {1365-2958},
  abstract =	 {Archaeal and eukaryotic organisms contain sets of C/D box 
                  s(no)RNAs with guide sequences that determine ribose 
                  2'-O-methylation sites of target RNAs. The composition of 
                  these C/D box sRNA sets is highly variable between organisms 
                  and results in varying RNA modification patterns which are 
                  important for ribosomal RNA folding and stability. Little is 
                  known about the genomic organization of C/D box sRNA genes 
                  in archaea. Here, we aimed to obtain first insights into the 
                  biogenesis of these archaeal C/D box sRNAs and analyzed the 
                  genetic context of more than 300 archaeal sRNA genes. We 
                  found that the majority of these genes do not possess 
                  independent promoters but are rather located at positions 
                  that allow for co-transcription with neighboring genes and 
                  their start or stop codons were frequently incorporated into 
                  the conserved boxC and D motifs. The biogenesis of 
                  plasmid-encoded C/D box sRNA variants was analyzed in vivo 
                  in Sulfolobus acidocaldarius. It was found that C/D box sRNA 
                  maturation occurs independent of their genetic context and 
                  relies solely on the presence of intact RNA kink-turn 
                  structures. The observed plasticity of C/D box sRNA 
                  biogenesis is suggested to enable their accelerated 
                  evolution and, consequently, allow for adjustments of the 
                  RNA modification landscape. This article is protected by 
                  copyright. All rights reserved.}
}