@Article{Tobias_Heinrich_Eresmann-Photo_symbi_depen-2016,
  author =	 {Tobias, Nicholas J. and Heinrich, Antje K. and Eresmann, 
                  Helena and Wright, Patrick R. and Neubacher, Nick and 
                  Backofen, Rolf and Bode, Helge B.},
  title =	 {Photorhabdus-nematode symbiosis is dependent on 
                  hfq-mediated regulation of secondary metabolites},
  journal =	 {Environ Microbiol},
  year =	 {2016},
  volume =	 {19},
  number =	 {1},
  pages =	 {119-129},
  user =	 {wrightp},
  pmid =	 {27555343},
  doi = 	 {10.1111/1462-2920.13502},
  issn = 	 {1462-2912},
  issn = 	 {1462-2920},
  abstract =	 {Photorhabdus luminescens maintains a symbiotic relationship 
                  with the nematodes Heterorhabditis bacteriophora and 
                  together they infect and kill insect larvae. To maintain 
                  this symbiotic relationship, the bacteria must produce an 
                  array of secondary metabolites to assist in the development 
                  and replication of nematodes. The regulatory mechanisms 
                  surrounding production of these compounds are mostly 
                  unknown. The global post-transcriptional regulator, Hfq, is 
                  widespread in bacteria and performs many functions, one of 
                  which is the facilitation of sRNA binding to target mRNAs, 
                  with recent research thoroughly exploring its various 
                  pleiotropic effects. Here we generate and characterize an 
                  hfq deletion mutant and show that in the absence of hfq, the 
                  bacteria are no longer able to maintain a healthy symbiosis 
                  with nematodes due to the abolishment of the production of 
                  all known secondary metabolites. RNAseq led us to produce a 
                  second deletion of a known repressor, HexA, in the same 
                  strain, which restored both metabolite production and 
                  symbiosis. This article is protected by copyright. All 
                  rights reserved.}
}