@Article{Richter_Lange_Backofen-CRISP_Compa_analy-2013,
  author =	 {Richter, Hagen and Lange, Sita J. and Backofen, Rolf and 
                  Randau, Lennart},
  title =	 {{Comparative} analysis of {Cas6b} processing 
                  and {CRISPR} {RNA} stability},
  journal =	 {RNA Biology},
  year =	 {2013},
  volume =	 {10},
  number =	 {5},
  pages =	 {700-707},
  doi = 	 {10.4161/rna.23715},
  user =	 {sita},
  pmid =	 {23392318},
  issn = 	 {1547-6286},
  issn = 	 {1555-8584},
  abstract =	 {The prokaryotic antiviral defense systems CRISPR (clustered 
                  regularly interspaced short palindromic repeats)/Cas 
                  (CRISPR-associated) employs short crRNAs (CRISPR RNAs) to 
                  target invading viral nucleic acids. A short spacer sequence 
                  of these crRNAs can be derived from a viral genome and 
                  recognizes a reoccurring attack of a virus via base 
                  complementarity. We analyzed the effect of spacer sequences 
                  on the maturation of crRNAs of the subtype I-B Methanococcus 
                  maripaludis C5 CRISPR cluster. The responsible endonuclease, 
                  termed Cas6b, bound non-hydrolyzable repeat RNA as a dimer 
                  and mature crRNA as a monomer. Comparative analysis of Cas6b 
                  processing of individual spacer-repeat-spacer RNA substrates 
                  and crRNA stability revealed the potential influence of 
                  spacer sequence and length on these parameters. Correlation 
                  of these observations with the variable abundance of crRNAs 
                  visualized by deep-sequencing analyses is discussed. 
                  Finally, insertion of spacer and repeat sequences with 
                  archaeal poly-T termination signals is suggested to be 
                  prevented in archaeal CRISPR/Cas systems.}
}