@Article{Overloper_Kraus_Gurski-Two_separ_modul-2014,
  author =	 {Overloeper, Aaron and Kraus, Alexander and Gurski, Rosemarie 
                  and Wright, Patrick R. and Georg, Jens and Hess, Wolfgang R. 
                  and Narberhaus, Franz},
  title =	 {Two separate modules of the conserved regulatory {RNA} 
                  {AbcR1} address multiple target {mRNAs} in and outside of 
                  the translation initiation region},
  journal =	 {RNA Biol},
  year =	 {2014},
  volume =	 {11},
  number =	 {5},
  pages =	 {624-40},
  doi = 	 {10.4161/rna.29145},
  user =	 {wrightp},
  pmid =	 {24921646},
  issn = 	 {1547-6286},
  issn = 	 {1555-8584},
  abstract =	 {The small RNA AbcR1 regulates the expression of ABC 
                  transporters in the plant pathogen Agrobacterium 
                  tumefaciens, the plant symbiont Sinorhizobium meliloti, and 
                  the human pathogen Brucella abortus. A combination of 
                  proteomic and bioinformatic approaches suggested dozens of 
                  AbcR1 targets in A. tumefaciens. Several of these newly 
                  discovered targets are involved in the uptake of amino 
                  acids, their derivatives, and sugars. Among the latter is 
                  the periplasmic sugar-binding protein ChvE, a component of 
                  the virulence signal transduction system. We examined 16 
                  targets and their interaction with AbcR1 in close detail. In 
                  addition to the previously described mRNA interaction site 
                  of AbcR1 (M1), the CopraRNA program predicted a second 
                  functional module (M2) as target-binding site. Both M1 and 
                  M2 contain single-stranded anti-SD motifs. Using mutated 
                  AbcR1 variants, we systematically tested by band shift 
                  experiments, which sRNA region is responsible for mRNA 
                  binding and gene regulation. On the target site, we find 
                  that AbcR1 interacts with some mRNAs in the translation 
                  initiation region and with others far into their coding 
                  sequence. Our data show that AbcR1 is a versatile master 
                  regulator of nutrient uptake systems in A. tumefaciens and 
                  related bacteria.}
}