@Article{Murakawa_Hinz_Mothes-post_regul_mRNA-2015,
  author =	 {Murakawa, Yasuhiro and Hinz, Michael and Mothes, Janina and 
                  Schuetz, Anja and Uhl, Michael and Wyler, Emanuel and 
                  Yasuda, Tomoharu and Mastrobuoni, Guido and Friedel, 
                  Caroline C. and Dolken, Lars and Kempa, Stefan and 
                  Schmidt-Supprian, Marc and Bluthgen, Nils and Backofen, Rolf 
                  and Heinemann, Udo and Wolf, Jana and Scheidereit, Claus and 
                  Landthaler, Markus},
  title =	 {{RC3H1} post-transcriptionally regulates {A20} {mRNA} and 
                  modulates the activity of the {IKK}/{NF}-{kappaB} pathway},
  journal =	 {Nat Commun},
  year =	 {2015},
  volume =	 {6},
  number =	 {},
  pages =	 {7367},
  user =	 {backofen},
  pmid =	 {26170170},
  doi = 	 {10.1038/ncomms8367},
  issn = 	 {2041-1723},
  abstract =	 {The RNA-binding protein RC3H1 (also known as ROQUIN) 
                  promotes TNFalpha mRNA decay via a 3'UTR constitutive decay 
                  element (CDE). Here we applied PAR-CLIP to human RC3H1 to 
                  identify approximately 3,800 mRNA targets with >16,000 
                  binding sites. A large number of sites are distinct from the 
                  consensus CDE and revealed a structure-sequence motif with 
                  U-rich sequences embedded in hairpins. RC3H1 binds 
                  preferentially short-lived and DNA damage-induced mRNAs, 
                  indicating a role of this RNA-binding protein in the 
                  post-transcriptional regulation of the DNA damage response. 
                  Intriguingly, RC3H1 affects expression of the NF-kappaB 
                  pathway regulators such as IkappaBalpha and A20. RC3H1 uses 
                  ROQ and Zn-finger domains to contact a binding site in the 
                  A20 3'UTR, demonstrating a not yet recognized mode of RC3H1 
                  binding. Knockdown of RC3H1 resulted in increased A20 
                  protein expression, thereby interfering with IkappaB kinase 
                  and NF-kappaB activities, demonstrating that RC3H1 can 
                  modulate the activity of the IKK/NF-kappaB pathway.}
}