@Article{Maticzka_Ilik_Aktas-uvCLA_fast_and-2018,
  author =	 {Maticzka, Daniel and Ilik, Ibrahim Avsar and Aktas, Tugce 
                  and Backofen, Rolf and Akhtar, Asifa},
  title =	 {{uvCLAP} is a fast and non-radioactive method to identify 
                  in vivo targets of {RNA}-binding proteins},
  journal =	 {Nat Commun},
  year =	 {2018},
  volume =	 {9},
  number =	 {1},
  pages =	 {1142},
  user =	 {maticzkd},
  pmid =	 {29559621},
  doi = 	 {10.1038/s41467-018-03575-4},
  issn = 	 {2041-1723},
  abstract =	 {RNA-binding proteins (RBPs) play important and essential 
                  roles in eukaryotic gene expression regulating splicing, 
                  localization, translation, and stability of mRNAs. We 
                  describe ultraviolet crosslinking and affinity purification 
                  (uvCLAP), an easy-to-use, robust, reproducible, and 
                  high-throughput method to determine in vivo targets of RBPs. 
                  uvCLAP is fast and does not rely on radioactive labeling of 
                  RNA. We investigate binding of 15 RBPs from fly, mouse, and 
                  human cells to test the method's performance and 
                  applicability. Multiplexing of signal and control libraries 
                  enables straightforward comparison of samples. Experiments 
                  for most proteins achieve high enrichment of signal over 
                  background. A point mutation and a natural splice isoform 
                  that change the RBP subcellular localization dramatically 
                  alter target selection without changing the targeted RNA 
                  motif, showing that compartmentalization of RBPs can be used 
                  as an elegant means to generate RNA target specificity.}
}