@Article{Maier_Stachler_Brendel-The_nuts_and-2019,
  author =	 {Maier, Lisa-Katharina and Stachler, Aris-Edda and Brendel, 
                  Jutta and Stoll, Britta and Fischer, Susan and Haas, Karina 
                  A. and Schwarz, Thandi S. and Alkhnbashi, Omer S. and 
                  Sharma, Kundan and Urlaub, Henning and Backofen, Rolf and 
                  Gophna, Uri and Marchfelder, Anita},
  title =	 {The nuts and bolts of the {Haloferax} {CRISPR}-{Cas} system 
                  {I}-{B}},
  journal =	 {RNA Biol},
  year =	 {2019},
  volume =	 {16},
  number =	 {4},
  pages =	 {469-480},
  user =	 {alkhanbo},
  pmid =	 {29649958},
  doi = 	 {10.1080/15476286.2018.1460994},
  issn = 	 {1547-6286},
  issn = 	 {1555-8584},
  abstract =	 {Invading genetic elements pose a constant threat to 
                  prokaryotic survival, requiring an effective defence. Eleven 
                  years ago, the arsenal of known defence mechanisms was 
                  expanded by the discovery of the CRISPR-Cas system. Although 
                  CRISPR-Cas is present in the majority of archaea, research 
                  often focuses on bacterial models. Here, we provide a 
                  perspective based on insights gained studying CRISPR-Cas 
                  system I-B of the archaeon Haloferax volcanii. The system 
                  relies on more than 50 different crRNAs, whose stability and 
                  maintenance critically depend on the proteins Cas5 and Cas7, 
                  which bind the crRNA and form the Cascade complex. The 
                  interference machinery requires a seed sequence and can 
                  interact with multiple PAM sequences. H. volcanii stands out 
                  as the first example of an organism that can tolerate 
                  autoimmunity via the CRISPR-Cas system while maintaining a 
                  constitutively active system. In addition, the H. volcanii 
                  system was successfully developed into a tool for gene 
                  regulation.}
}