@Article{Liao_Vogel_Hauber-CdrS_Globa_Trans-2021,
  author =	 {Liao, Yan and Vogel, Verena and Hauber, Sabine and Bartel, 
                  Jurgen and Alkhnbashi, Omer S. and Maass, Sandra and 
                  Schwarz, Thandi S. and Backofen, Rolf and Becher, Dorte and 
                  Duggin, Iain G. and Marchfelder, Anita},
  title =	 {{CdrS} {Is} a {Global} {Transcriptional} {Regulator} 
                  {Influencing} {Cell} {Division} in {Haloferax} volcanii},
  journal =	 {mBio},
  year =	 {2021},
  volume =	 {12},
  number =	 {4},
  pages =	 {e0141621},
  user =	 {backofen},
  pmid =	 {34253062},
  doi = 	 {10.1128/mBio.01416-21},
  issn = 	 {2150-7511},
  abstract =	 {Transcriptional regulators that integrate cellular and 
                  environmental signals to control cell division are well 
                  known in bacteria and eukaryotes, but their existence is 
                  poorly understood in archaea. We identified a conserved gene 
                  (cdrS) that encodes a small protein and is highly 
                  transcribed in the model archaeon Haloferax volcanii. The 
                  cdrS gene could not be deleted, but CRISPR interference 
                  (CRISPRi)-mediated repression of the cdrS gene caused slow 
                  growth and cell division defects and changed the expression 
                  of multiple genes and their products associated with cell 
                  division, protein degradation, and metabolism. Consistent 
                  with this complex regulatory network, overexpression of cdrS 
                  inhibited cell division, whereas overexpression of the 
                  operon encoding both CdrS and a tubulin-like cell division 
                  protein (FtsZ2) stimulated division. Chromatin 
                  immunoprecipitation-DNA sequencing (ChIP-Seq) identified 18 
                  DNA-binding sites of the CdrS protein, including one 
                  upstream of the promoter for a cell division gene, ftsZ1, 
                  and another upstream of the essential gene dacZ, encoding 
                  diadenylate cyclase involved in c-di-AMP signaling, which is 
                  implicated in the regulation of cell division. These 
                  findings suggest that CdrS is a transcription factor that 
                  plays a central role in a regulatory network coordinating 
                  metabolism and cell division. IMPORTANCE Cell division is a 
                  central mechanism of life and is essential for growth and 
                  development. Members of the Bacteria and Eukarya have 
                  different mechanisms for cell division, which have been 
                  studied in detail. In contrast, cell division in members of 
                  the Archaea is still understudied, and its regulation is 
                  poorly understood. Interestingly, different cell division 
                  machineries appear in members of the Archaea, with the 
                  Euryarchaeota using a cell division apparatus based on the 
                  tubulin-like cytoskeletal protein FtsZ, as in bacteria. 
                  Here, we identify the small protein CdrS as essential for 
                  survival and a central regulator of cell division in the 
                  euryarchaeon Haloferax volcanii. CdrS also appears to 
                  coordinate other cellular pathways, including synthesis of 
                  signaling molecules and protein degradation. Our results 
                  show that CdrS plays a sophisticated role in cell division, 
                  including regulation of numerous associated genes. These 
                  findings are expected to initiate investigations into 
                  conditional regulation of division in archaea.}
}