@Article{Alkhnbashi_Meier_Mitrofanov-CRISP_bioin-2019,
  author =	 {Alkhnbashi, Omer S. and Meier, Tobias and Mitrofanov, 
                  Alexander and Backofen, Rolf and Voss, Bjorn},
  title =	 {{CRISPR}-{Cas} bioinformatics},
  journal =	 {Methods},
  year =	 {2019},
  volume =	 {},
  number =	 {},
  pages =	 {},
  user =	 {alkhanbo},
  pmid =	 {31326596},
  doi = 	 {10.1016/j.ymeth.2019.07.013},
  issn = 	 {1095-9130},
  issn = 	 {1046-2023},
  abstract =	 {Clustered regularly interspaced short palindromic repeats 
                  (CRISPR) and their associated proteins (Cas) are essential 
                  genetic elements in many archaeal and bacterial genomes, 
                  playing a key role in a prokaryote adaptive immune system 
                  against invasive foreign elements. In recent years, the 
                  CRISPR-Cas system has also been engineered to facilitate 
                  target gene editing in eukaryotic genomes. Bioinformatics 
                  played an essential role in the detection and analysis of 
                  CRISPR systems and here we review the bioinformatics-based 
                  efforts that pushed the field of CRISPR-Cas research 
                  further. We discuss the bioinformatics tools that have been 
                  published over the last few years and, finally, present the 
                  most popular tools for the design of CRISPR-Cas9 guides.}
}